ALDOA anticorps (HRP)

N° du produit ABIN7565724

Cet anticorps anti-ALDOA est un anticorps Chèvre Polyclonal détectant ALDOA dans WB, ELISA et IP. Adapté pour Lapin.

Aperçu rapide pour ALDOA anticorps (HRP) (ABIN7565724)

Antigène: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ALDOA est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-ALDOA anticorps (HRP)

Fonction: Aldolase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle].

Attributs du produit: Synonyms: goat anti-Aldolase Antibody, HRP conjugated goat anti-Aldolase Antibody, Fructose-bisphosphate aldolase A, Muscle-type aldolase, peroxidase Aldolase

Purification: Aldolase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Aldolase [Rabbit Muscle]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Aldolase has been tested by ELISA, immunoprecipitation, and western blot. This product is assayed against 1.0 μg of Aldolase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur ALDOA

Antigène: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Autre désignation: ALDOA

Sujet: Background: Aldolase is an enzyme that performs an aldol reaction (creating an aldol) or its reverse (cleaving an aldol). Aldolase plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein. Belongs to the class I fructose-bisphosphate aldolase family.

ID gène: 100009055

NCBI Accession: NP_001075707

UniProt: P00883

Pathways: Ribonucleoside Biosynthetic Process